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Géraldine Guasch (CRCM) - Publication in Nature Communications -

The flow cytometry and cell sorting platform

Flow cytometry allows the quantitative analysis of microscopic particles, such as cells and chromosomes, by suspending them in a stream of fluid and passing them by an electronic laser detection apparatus.

It allows simultaneous multiparametric analysis of the physical and/or chemical characteristics of up to thousands of particles per second. Flow cytometry is routinely used in the diagnosis of health disorders, especially blood cancers, but has many other applications in both research and clinical practice.
This technology also allows the physical sorting of particles based on their properties, so as to purify populations of interest. The flow cytometry platform of CRCM proposes technical tools, assistance and a technological watch to research teams in order to keep the CRCM’s projects at highest technological level.

The staff of the technical platform cytometry: Manon Richaud (manager) and Françoise Mallet (technician). The scientific responsibility is ensured by Daniel Olive. The service is supported by a steering committee consisting of representatives of the research team, an administrative representative and the Director or his representative.

 

Platform equipments
Cell sorting

2 sorting cytometers (Becton Dickinson) :

- Aria II (3 lasers 405 /488/633nm - 9 PMT)
- Aria III SORP (5 lasers 350/405/488/532/630 nm - 18 PMT)

Analyzers

4 analyzing cytometers :

- LSRII SORP analyzer (4 lasers 405/488/532/630- 17 PMT) (Becton Dickinson)
- Fortessa analyzer (3 lasers 405/488/630- 11 PMT) (Becton Dickinson)
- Canto analyzer (2 lasers 488/630 - 6 PMT) (Becton Dickinson)

Automacs

Automacs Pro (Miltenyi Biotec) under hood

BIO-PLEX

The platform is equipped with a BIO-Plex 200 (BioRad)

Softwares

1 workstation (PC) equipped with FlowJo and Spectroflo software.

1 workstation (PC) equipped with KALUZA, FlowJo and Diva software.

1 OMIQ license

Education and Training