In Vitro, In vivo models

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In Vitro, In vivo models
cell culture platform

Location: TPR2 building, Marseille-Luminy Campus

The Cell Culture Platform includes four laboratories allowing the use and handling under the best conditions of organisms belonging to biological risk class 2, as well as a satellite laboratory that houses equipment used in cell biology (electroporation units, cell counters, plate readers, fluorescence digital microscope, etc.).
It is divided into affected areas according to technical requirements and biological risk, which are accessible through an online reservation procedure.

Mission Statement
The Platform's mission is to provide the CRCM, the TPR2 research units and their collaborators with facilities and services for the culture of eukaryotic cells, according to the highest quality standards. It can potentiate the collaborations established with university research structures or with private structures, both nationally and internationally.

Services
Provision of a fully controlled cell culture space, and in compliance with Good Cell Culture Practices
Cell culture training
Participation in research projects using cell culture
Technical expertise
Technological monitoring

How it works
The organization of the Platform is coordinated by an ITA from U1068. The Quality Management System complies with ISO9001. The objective is to achieve continuous improvement of the service to users in order to meet their expectations, in compliance with laws and regulations.

An Internal Regulation sets out the rules for using the Platform (competence of the experimenters, equipment reservation, equipment maintenance, waste management, etc.).

The operating budget is subject to a Use Agreement.

The animal house provides basic services for the breeding of animals from wild or genetically modified animals. The animals are kept in EOPS protected areas (free of specific pathogenic organisms). Most mouse strains are kept in individual ventilated cages and handled under sterile conditions. The dedicated staff provides qualified technical assistance for genotyping and injection of ES cells.

IPC SITE :

The animal house covers an area of 300m2 divided into 5 rooms: two A2 accommodation areas with a capacity of 1500 cages, an experimentation area with a capacity of 600 cages, a transgenesis area and a laboratory equipped with a bioluminescence and intra-vital fluorescence system (Photon Imager, BioSpace Lab) with respective accommodation capacities of 200 cages. The experimental area is equipped with state-of-the-art equipment for recording hematological parameters. In vivo imaging services are available through the TrGET platform.
The CRCM animal facility is part of a network of animal facilities shared by two Federal Research Institutes: Cancer and Immunology and the Institut de Biologie de Développement de Marseille.

Luminy site:
The PSEA (Platform for Stabling and Animal Experimentation) animal house at the Luminy site covers 250 m2. The surface is divided into 4 A1+ housing/experimentation areas (including one specifically used for aseptic hysterectomy rederivation) and one A2 housing/experimentation area. These areas can currently contain 700 and 244 cages respectively, with the ability to increase A1+ areas from 700 to 1200 cages. The surface also includes an autoclaved equipment storage area for A1+ zones and an autoclaved equipment storage area for A2 zone, a circulation area, as well as a 30m2 experimental laboratory equipped in accordance with the technical needs of users (including a bioluminescence and intra-vital fluorescence system with BioSpaceLab Photon Imager). This animal house is governed by strict internal regulations where the main animal housing and testing procedures are predefined. It is managed by a technical manager/personnel manager and a scientific/technical advisor. In coordination with current regulations, a pharmacy manager and a "well-being" committee are also involved in the daily operations.
The PSEA animal facility at the Luminy site is also part of a network of animal facilities shared by two Federal Research Institutes: Cancer and Immunology and the Institut de Biologie de Développement de Marseille.

The transgenesis laboratory is intended to produce mice genetically modified by insertion mutagenesis or by Recombination Homologue for the various teams at the Centre. It includes an injection station composed of an inverted Leïca microscope coupled with 2 micromanipulators and 2 micropumps.

It is also equipped with a laminar flow hood and a CO2 incubator for the culture of ES cells in a mycoplasma-free context. It also offers workspaces with 2 binocular magnifiers for the study of mutant mice. The transgenesis department manages ES cells and primary fibroblasts that are neo-resistant and the various environments required.

It offers users training and/or guidance for ES cell culture and Homologous Recombination (vector construction, electroporation and selection of ES cells with RH). It offers links with the various European and global banks that offer genetically modified ES cells. The transgenesis department carries out the different injection steps from the ES cells provided by the users, i.e. blastocyst sampling, micro-injection of the ES cells and re-implantation in the surrogate mother. It manages the animals required for injections (sterilization of males, management of fertile animals, mating) and then the establishment of lines from the animal house.