The Marseille Cancer Research Center celebrates its 50th anniversary ! -
The Microscopy and Scientific Imaging platform is essentially organized around light microscopy techniques: wide-field, confocal and structured light microscopy, etc.
It offers a coherent set of materials which allows work using different exploration methods: either on cells or tissue fragments fixed on fluorescent or colored samples, or on samples studied dynamically, whether via fluorescence techniques or phase contrast.
This platform is shared by all CRCM teams and opened to the scientific community. It provides training and support to all users, maintains and upgrades materials and software, develops analytical tool and performs technological watch.
The platform is supervised by a research scientist, managed by an engineer and includes the following equipment:
LSM 880 confocal microscope (Zeiss) with an Airyscan resolution enhancement module. A confocal microscope AX NIKON with a FRAP UVA (365nm) module. A confocal multiple-point microscope with a « spinning disk », a FRAP, FLIP, Phot-Activation system, able to operate in TIRF mode. A fluorescence structured illumination microscope – Apotome (Zeiss) with a color camera for colored samples. 2 time-lapse fluorescence microscopes (Zeiss-Roper Scientifics and Olympus). A fluorescence microscope equipped for molecular combing. A Genomic vision fluorescence slide scanner (12 slots). Computer stations for image processing and analysis.
The platform is constantly evolving to try to best adapt to the reality of projects and user needs.
Excitation wavelengths: a UV diode (405 nm), 3 visible lasers (a multi-line argon laser at 458 nm, 488 nm, and 514 nm, a DPSS at 561 nm, and a HeNe at 633 nm).
The microscope is equipped with a temperature-controlled chamber allowing the acquisition of live samples over time.
The spectral analysis module can be used for the subtraction of autofluorescence or the separation of fluorochromes with overlapping excitation and emission spectra.
The Airyscan module improves resolution.
Confocal AX NIKON + FRAP UV
NIKON AX confocal + FRAP UVA module, 1 HS-PMT + 3 GaAsP, inverted and motorized stand + focus maintenance system.
