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Mitochondria, potential new therapeutic targets in pancreatic cancer -

3D-Hub-O

INTRODUCTION

Advances in developmental and stem cell biology has allowed to decipher the growth factors and signaling pathways necessary for a stem cell to receive proper environmental conditions to differentiate and arrange themselves to form a mini-organ in vitro. These three-dimensional (3D) structures called ‘’organoids’’ display similar functional properties of the tissue of origin including the genetic and the cellular heterogeneity, making them highly valuable tools to study tissue homeostasis and tumorigenesis.

GOAL

– The use of this three-dimensional culture system to model developmental processes in normal and disease states.

– To recapitulate early stage of cancer formation and to create new cellular models of tumor directly from patient biopsies which represent the cellular and genetic heterogeneity of cancer better than any immortalized cell lines.

– To functionally test the pathways by which given cells participate in tissue maintenance and remodelling, to dissect the mechanisms underlying tumorigenesis and to test drug response in different cancers paving the way to personalized medicine.

 

DIRECTION

Scientific manager : Géraldine Guasch

Technical manager : Véronique Chevrier

Date of creation : 2020

Location : IPC

Satff : 1 engineer (Alexane Ollivier)

The Hub-O platform is labeled by the Canceropole PACA

PLATFORM EQUIPMENTS

Stéréomicroscope Leica M205FA motorized under sterile hood. DAPI, GFP, YFP, RFP filters and monochrome Leica DFC9000 GT camera. Logiciel Leica LAS X.

Motorized Microscope Evos M7000 with CELESTE software

Electroporator Sonidel NEPA21 specialized for organoids electroporation

Cell culture basis (Incubator HERAcell VIOS 160i, Sterile hood, Centrifugeuse…)