La protéine PLZF et le vieillissement des cellules souches hématopoïétiques

Hematopoietic stem cells (HSCs) ensure the life-long production of all blood lineages thanks to their ability to selfrenew and differentiate into mature cells. Because they have to persist throughout an organism’s lifespan, HSCs need to remain protected from damage and exhaustion. We know that the HSC pool is heterogeneous and not all HSCs react in the same way to the environmental stress, so it is important to work at the single cell level. We previously showed that the promyelocytic zinc finger (PLZF) transcription factor is involved in HSC physiology and transcriptome analysis at the single cell level revealed that PLZF activity protects from aging deterioration a subpopulation of HSCs. This may be mediated by its epigenetic activity at enhancer regions that control genes involved in metabolism and inflammation. The M2 student will be involved in a project aiming to understand the interaction between, epigenetics, metabolism and aging of HSCs at the single cell level.