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Le Centre de Recherche en Cancérologie de Marseille fête ses 50 ans ! -

10 2019 Progress in biophysics and molecular biology

Plugged into the Ku-DNA hub: The NHEJ network.


Frit P, Ropars V, Modesti M, Charbonnier JB, Calsou P


In vertebrates, double-strand breaks in DNA are primarily repaired by Non-Homologous End-Joining (NHEJ). The ring-shaped Ku heterodimer rapidly senses and threads onto broken DNA ends forming a recruiting hub. Through protein-protein contacts eventually reinforced by protein-DNA interactions, the Ku-DNA hub attracts a series of specialized proteins with scaffolding and/or enzymatic properties. To shed light on these dynamic interplays, we review here current knowledge on proteins directly interacting with Ku and on the contact points involved, with a particular accent on the different classes of Ku-binding motifs identified in several Ku partners. An integrated structural model of the core NHEJ network at the synapsis step is proposed.

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