Mar 2014 Small (Weinheim an der Bergstrasse, Germany)

Probing physical properties of a DNA-protein complex using nanofluidic channels.

Auteurs

Frykholm K, Alizadehheidari M, Fritzsche J, Wigenius J, Modesti M, Persson F, Westerlund F

Résumé

A method to investigate physical properties of a DNA-protein complex in solution is demonstrated. By using tapered nanochannels and lipid passivation the persistence length of a RecA filament formed on double-stranded DNA is determined to 1.15 μm, in agreement with the literature, without attaching protein or DNA to any handles or surfaces.

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